Occasionally it causes opportunistic infections, a. Contrary to previous findings for the lysis of gramnegative bacteria, prior removal of the outer membrane was not essential for enzymic lysis. Production of biodegradable copolyesters by alcaligenes eutrophus. Pha polymers were accumulated in the cells in nitrogenfree mineral media containing various carbon substrates, and the accumulated pha polymers were subsequently degraded after the carbon sources were exhausted. Alcaligenes eutrophus and its role in biodegradable. Citeseerx production of phb and phbcohv biopolymers. Get a printable copy pdf file of the complete article 1. Madyastha, kattigere m and gururaja, tarikere l 1994 asymmetric reduction of prochiral ketones bv cellfree systems from alcaligenes eutrophus. Polybetahydroxybutyrate phb biosynthesis in alcaligenes.
Read catabolism of 2,6dinitrophenol by alcaligenes eutrophus jmp 4 and jmp 222, archives of microbiology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Be sure to italicize all mentions of specific species names, such as e. Biopolymers were produced by using alcaligenes eutrophus microorganisms. Stanier departmentofbacteriology andimmunology, university ofcalifornia, berkeley, california 94720 received for publication 2 april 1971 the range ofaromatic compounds that support the growth ofalcaligenes eutrophus has been determined, and the pathways used for the dissimilation of these. Mutants of alcaligenes eutrophus defective in autotrophic metabolism. Alcaligenes faecalis causes nosocomial sepsis, arising from contaminated hemodialysis or intravenous fluid, in immunocompromised patients alcaligenes species have been used for the industrial production of nonstandard amino acids. Poly3hydroxybutyrate p3hb was produced by alcaligenes eutrophus from fructose or butyric acid in nitrogenfree media at different phs and temperatures, and the timedependent changes in the molecular weight of p3hb were studied. Metal ion uptake by a plasmidfree metalsensitive alcaligenes eutrophus strain article pdf available in journal of bacteriology 1717. Alcaligenes eutrophus was grown in continuous culture 34 c, ph 6. Batch production of polyhydroxybutyrate phb by ralstonia.
In alcaligenes eutrophus hi6 the hyp gene complex consists of six open reading frames hypal, bl, fi, c, d and e whose products are involved in maturation of the two nife hydrogenases. Asymmetric reduction of prochiral ketones bv cellfree. Alcaligenes definition of alcaligenes by medical dictionary. Read separation of alcaligenes eutrophus cells containing poly3. Mutants of alcaligenes eutrophus defective in autotrophic. Some of substrates such as glucose, sucrose, butyric, pentanoic and propanoic acids used as carbon sources affect the accumulation and composition of biopolymers in the cell.
In alcaligenes eutrophus ch34, determinants encoding inducible resistance to chromate chr and to cobalt and nickel cnr are located adjacent to each other on plasmid pmol28. Cloning of the alcaligenes eutrophus genes for synthesis of polybetahydroxybutyric acid phb and synthesis of phb in escherichia coli. Degradation of trichlorophenols by alcaligenes eutrophus jmp4. Cupriavidus metallidurans strain ch34 renamed from ralstonia metallidurans and previously known as ralstonia eutropha and alcaligenes eutrophus is a nonsporeforming, gramnegative bacterium which is adapted to survive several forms of heavy metal stress. The facultatively chemolithoautotrophic hydrogen bacterium alcaligenes eutrophus was able to utilize organic and inorganic substrates concomitantly, i.
The use of cytophaga lysing enzymes was investigated for the liberation of poly. Characterization of the active site of a hydrogen sensor. The nirs product showed extensive homology with periplasmic nitrite. A novel multicomponent regulatory system mediates h2. Pdf degradation of trichlorophenols by alcaligenes.
Proposed pathway for the degradation of nerolidol by alcaligenes eutrophus. Complete cell lysis was approached within a 60 minute period. Recovery and characterization of poly3hydroxybutyric. Oxidation of molecular hydrogen catalyzed by nife hydrogenases is a widespread mechanism of energy generation among prokaryotes. Genetic and physical mapping and expression in pseudomonas aeruginosa of the chromosomally encoded ribulose bisphosphate carboxylase genes of alcaligenes eutrophus. If you are interested, you may visit the project page and join with us. In alcaligenes eutrophus h16 the hyp gene complex consists of six open reading.
Cloning of the alcaligenes eutrophus genes for synthesis. Alcaligenes eutrophus, biodegradation, nerolidol, new oxidative pathway, oxidoreductase, asymmetric reduction, terpenoids and steroids. Plasmidfree cells of strain ch34 are still able to grow lithoautotrophically and to form both. Catabolism of 2,6dinitrophenol by alcaligenes eutrophus. Cupriavidus metallidurans strain ch34, renamed from ralstonia metallidurans and previously known as ralstonia eutropha and alcaligenes eutrophus is a nonsporeforming, gramnegative bacterium which is adapted to survive several forms of heavy metal stress. The lysis of gramnegative alcaligenes eutrophus by. Polyphydroxybutyrate phb biosynthesis in alcaligenes eutrophus h16 identification and characterization of the phb polymerase gene phbc. Alcaligenes strains were grown in mineral salts medium containing 0. Asymmetric reduction of prochiral ketones by cellfree. Selective transport of divalent cations by transition.
Alcaligenes eutrophus strain ch34, which was isolated as a bacterium resistant to cobalt, zinc, and cadmium ions, shares with a. Separation of alcaligenes eutrophus cells containing poly. Feel free to leave messages at the project talk page. Metal ion uptake by a plasmid free metalsensitive alcaligenes eutrophus strain article pdf available in journal of bacteriology 1717. Asvmmetric reduction of prochiral ketones bv j cellfree. Synthesis and degradation of polyhydroxyalkanoates in alcaligenes. The journal of biological chemistry 0 1989 by the american society for biochemistry and molecular biology, inc. Pha polymers were accumulated in the cells in nitro genfree mineral media containing various carbon substrates. Biological removal of cadmium by alcaligenes eutrophus ch 34. B could be adapted to 2fluorobenzoate as the sole source of carbon and energy. Pdf alcaligenes eutrophus strain ch34, which was isolated as a bacterium resistant.
Denitrification by alcaligenes eutrophus h16 is genetically linked to megaplasmid phg1. Alcaligenes eutrophus as a model system for bacterial interactions with heavy metals in the environment. Synthesis and degradation of polyhydroxyalkanoates in. Production of phb and phbcohv biopolymers by using. Cultures were har vested by centrifugation in the cold 4 c. The mixotrophic capabilities were investigated under succinatelimited growth of a. A strain of alcaligenes eutrophus has been isolated from the soil by enrichment culture technique with nerolidol 1, a sesquiterpene alcohol, as the sole source of carbon and energy. Phb production by alcaligenes eutrophus growing in continuous. Plasmidfree cells of strain ch34 are still able to grow. Cyclic nature of poly3hydroxyalkanoate metabolism in. Fermentation of nerolidol 1 by this bacterium in a mineral salts medium resulted in the formation of two major metabolites, viz. Enzyme assays all photometric measurements were carried out.
Citeseerx document details isaac councill, lee giles, pradeep teregowda. The cyclic process of biosynthesis and degradation of poly3hydroxyalkanoate pha was studied in alcaligenes eutrophus under conditions of nitrogenlimitation of growth. Alcaligenes eutrophus h 16 wilde, 1962 atcc 17699, dsm428. Three strains of hydrogenutilizing bacteria were found to constitute a new species which we propose to assign to the genus alcaligenes with the name alcaligenes latus sp. Unexpectedly, the gene encoding the nitrite reductase nirs was identified on chromosomal dna. Biological removal of cadmium by alcaligenes eutrophus ch 34 1a. Pdf alcaligenes eutrophus ch34 is a facultative chemolithotroph. To develop metalsensing bacterial strains, a cloned part of plasmid pmol28, which contains both determinants, was mutated with tn 5lacz. Diels 1department of environmental health engineering, school of public health, center for environmental research, tehran university of medical sciences, tehran, iran 2felemish institute of technological research, v. A random copolymer of 3hydroxybutyrate and 3hydroxypropionate, p3hbco 3hp, was produced in a nitrogenfree culture solution of 3hydroxypropionic acid. In this report we describe a novel signal transduction system regulating hydrogenase gene hox expression. A physiologically based metabolic model for growth and polybetahydroxybutyrate phb production in alcaligenes eutrophus h16 has been formulated. A novel multicomponent regulatory system mediates h. Characterization of the active site of a hydrogen sensor from alcaligenes eutrophus antonio j.
Identification and characterization of the phb polymerase gene phbc. Dissimilation of aromaticcompounds by alcaligenes eutrophus. Biosynthesis of the h2oxidizing enzymes is a complex process subject to positive control by h2 and negative control by organic energy sources. Structural gene nirs for the cytochrome cd1 nitrite. Alcaligenes eutrophus strain ch34, which was isolated as a bacterium. Alcaligenes eutrophus ch34 is a facultative chemolithotroph with. Alcaligenes faecalis a bacillus which is a motile, nonnitrate reducing, oxidase positive, catalase positive, and citrate positive gramnegative obligate aerobe that colonises humid areas in hospitals and may transiently colonise the skin. Other articles where alcaligenes eutrophus is discussed. Alcaligenes article about alcaligenes by the free dictionary. Other roles of alcaligenes eutrophus bioremediation agent. Alcaligenes eutrophus have developed resistance to heavy metal ions through mutations and hence can grow in the presence those otherwise deadly ions. Alcaligenes eutrophus as a bacterial chromate sensor. P3hb polymers were accumulated within cells in the presence of a carbon source, and the rate of p3hb accumulation increased with temperature in the.
Alcaligenes faecalis definition of alcaligenes faecalis. The cell free extract carried out both oxidation and reduction reactions at two different ph values and exhibited wide substrate specificity towards various steroids besides terpenes. Us5518907a cloning and expression in escherichia coli of. Pdf metal ion uptake by a plasmidfree metalsensitive. The phenotypic properties of this species are described, and a minor modification of the definition of alcaligenes is proposed. Although the transporters are highly similar, different preferences for divalent. Polybetahydroxybutyrate phb biosynthesis in alcaligenes eutrophus h16. Alcaligenes eutrophus is within the scope of wikiproject polymers which aims to improve the coverage of polymerrelated articles in wikipedia. Alcaligenes latus, a new species of hydrogenutilizing. The kinetics and mechanism of the synthesis and degradation of polyhydroxyalkanoates pha in alcaligenes eutrophus have been studied. Therefore, it is an ideal subject to study heavy metal disturbance of cellular processes. Based coagulants, biotechnology progress on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
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